Card games: The final frontier. These are the voyages of the Starship Kuriboh. Its five year mission: To abridge strange and confusing duels. To seek out new gags and pop culture references. To boldly go where no limey has gone before. In this episode, Yami seduces another greenskinned alien chick. Or maybe he just duels a mime. It's hard to say.
The episode's title is a parody of The Best of Both Worlds, the two-part episode of Star Trek: The Next Generation that served as the cliffhanger between Seasons 3 and 4 of that series. The introduction summary ("Last time on Star Trek: The Next Generation...") of Part II of The Best Of Both Worlds was used as the source footage for the pre-credit sequence of this episode; most of the dialogue used is the same as in the source footage, only overdubbed with appropriate TAS character voices.
While riding on his ship, Marik sings the theme song to the TV show "Gilligan's Island" and refers to two of the show's main characters, Gilligan and the Skipper.
The sound effect used during Strings' jump is the same one used for Steve Austin, thus reinforcing Strings' membership in the family Steve.
Yami says Yugi only thinks vampires can go out in daylight from reading the Twilight books, a series of young adult novels in which vampires are the heroes.
The line "Despite all my rage, I am still just a rat in a cage" is the chorus lyric to the song "Bullet with Butterfly Wings" by The Smashing Pumpkins.
The sounds made by Slifer are voice clips of Sinistar, from the arcade game of the same name.
Yami references an interview with Sony executive Kazuo Hirai, in which he said he'd tried playing the Nintendo Wii and "it got boring after about five minutes".
Joey's line "That's how I roll." is a reference to pro wrestler Christian, whose catchphrase is "And that's... how I roll."
In the final scene LittleKuriboh tries playing "More Than a Feeling" by Boston to see if it will make Marik on a motorcycle look cool.